Mycorrhizal Diversity of Acaulosporaceae and Glomaceae Families in the Sorghum Rhizosphere in Brazilian Acid Soils

摘要

The genetic diversity of arbuscular mycorrhizal fungi ( AMF) was characterized in the rhizosphere of two sorghum genotypes contrasting for tolerance to aluminum toxicity, using molecular techniques. The plants were cultivated in an oxisol of the Brazilian cerrado with three levels of aluminum saturation (0, 20% and 40% ). DNA samples were amplified based on nested PCR using fungal universal primers and specific primers for Glomaceae and Acaulosporaceae families. DGGE profiles clearly revealed that the diversity (number of amplicons) found was higher for Acaulosporaceae than Glomaceae. Both the sorghum cultivar and the level of aluminum saturation affected the AM fungi community structure.