Study on cloning and expression of HCV recombinant core protein

摘要

Core antigen of hepatitis C virus (HCV) is one of the important antigens to detect anti-HCV antibodies. In the present study, RTPCR was used to clone core gene fragment from the blood of a Chinese HCV infected patient. The cloned gene was inserted into the plasmid vector pET28a(+), and the recombinant plasmid pEC4 was transformed into E.coli BL21(DE3). After inducing by IPTG, SDS-PAGE analysis showed the expressed core protein was 15