CONSTRUCTION OF ASYMMETRIC CELL-SIZED LIPID VESICLES FROM LIPID-COATED WATER-IN-OIL MICRODRQPLETS

摘要

We present a simple, rapid, and robust method for preparing asymmetric cell-sized lipid bilayer vesicles using water-in-oil (W/O) microdroplets transferred through an oil-water interface. The efficiency for producing cell-sized model membranes is elucidated in relation to the vesicular size and the weight of contained watersoluble molecules. We demonstrate the biological asymmetric nature and the formation of lipid raft microdomain structures using fluorescence microscopy.