THE HARD SEGMENT OF POLYURETHANE WITH THE TUMOR-PROMOTING ACTIVITY MEDIATED BY CONNEXIN 43 AND ITS PHOSPHORYLATION ON V79 CELL LINE

摘要

In this investigation we studied the roles of the connexin 43 and its phosphorylation form, a major intercellular communication protein, in the tumorigenesis induced by hard segment of polyurethane. The hard segment, 4,4'-di (ethoxycarboamide) diphenymethane (MDU), significantly reduce the quality of connexin 43 and mainly increase the connexin 43 phosphorylation on serine, slightly increase the phosphorylation on tyrosine and threonine. PU4 and PU8 decrease the amount of connexin 43 and increase the connexin 43 phosphorylation on serine and theonine. The gap junctional permeability measured with Lucifer Yellow also shown that the MDU reduce the cell-cell communication. The results indicated that the hard segment of polyurethane have the tumor-promoting activity and mediated by the connexin 43 and its phosphorylation.