Antitumor Efficacy of Electrospun Hydroxyapatite Nanoparticles/poly (lactic-co-glycolic acid) Composite Nanofibers Encapsulated with Doxorubicin

摘要

The utility of nanofibrous electrospun composite scaffolds has attracted much interest in serving as drug delivery vehicles in recent years.In this present study,we attempted to first encapsulate an anticancer drug,doxorubicin (DOX) using an inorganic rod-like nano-hydroxyapatite (n-HA).Then,the DOX-loaded n-HA particles were mixed with poly(lactic-co-glycolic acid) (PLGA) solution to fabricate electrospun hybrid nanofibers.The formed drug-loaded composite nanofibers were characterized using scanning electron microscopy (SEM),transmission electron microscopy (TEM),laser scanning confocal microscope (LSCM) and mechanical testing.In vitro DOX release behavior was examined using UV-Vis spectroscopy at neutral and acidic conditions,respectively.We show that DOX is able to be successfully loaded onto the surface of the n-HA and the formed composite fibers have a uniform and continuous fibrous morphology.Importantly,the loaded DOX showed a sustained release profile as well as a significant improvement of mechanical durability.With the reduced burst-release profile,the organic/inorganic hybrid nanofibers may be able to be used as a drug delivery carrier for encapsulation and sustained release of different drugs for different biomedical applications.