Detection of E.coli O157∶H7 from Local Beef Meat Products and Poultry Meat Products in Duhok Province Markets using Conventional Culture and PCR

摘要

The study included detection of Escherichia coli 0157∶ H7 in local beef and poultry meats represented with minced meat,beef burger and kebab for beef while the whole chicken,chicken burger,drum stick,breast and wings represented poultry meat samples,during the period from November 2011 till June 2012.Detection of these organisms depends on culture characteristic of colonies and biochemical tests.as well as using of polymerase reaction assay (PCR)as recent technique for confirmation.The serotyping depends on the presence of the gens rfbO157 andflicH7.Out of the total 100 sample,E.coli was isolated rfb0157 gene in I sample (2％ )of local poultry rueat while beef samples was negative for this gene.Also we detected flicH7 gene in 2 samples (4％ )in imported beef whereas poultry meat revealed this gene (2％).DNA concentration of E.coli strains isolated from beef and poultry meat was between 67.7ng/μl and 53.8 ng/μl respectively.We attained specific duplicated band with molecular weight 625 bp for flicH7 gene while rfbO157 gene revealed 259 bp.