Black Tea Waste (Bohea Bulu)as Defaunation Agent on Methane Reduction of Rumen Fermentation in Enhancing Friendly Environment of Animal Production

摘要

This experiment was conducted to determine the effect of black tea waste as a defaunation agent on ruethane production,during in vitro rumen fermentation.Bohea bulu contains 2.95％ tannin which can be used as defaunation agent.The treatments in this experiment were tannin levels of 0 (T1),3 (T2),6 (T3),and 12 (T4)mg/g dry matter (DM)of feed.A combination of king grass: rice bran (70∶ 30)was used as the substrate.Each treatment consisted of three replications.Fermentations.ruonitored by the gas production technique,were carried out for 72 hours at 39℃.The data were analyzed using ANOVA and the differences of the means were compared by Duncan's new multiple range test (DMRT).The results showed that addition of bohea bulu equal to tannin levels of 3,6,and 12 mg/g DM of feed decreased protozoa number (P＜0.05)by 27.61％,34.96％ and 72.93％ and also decreased methane production as well as 27.69％,62.36％ and 70.61％ respectively compared to the control.Bohea bulu addition did not affect microbial protein production and pH,but NH3 concentrations declined (P＜0.05)as much as 27.54％,28.86％,and 39.95％ compared to the control.From the results it could be concluded that the addition of Bohea bulu equal to tannin up to 6 mg/g DM of feed could be used to reduce methane production without detrimental affect on the rumen fermentation.