A MICROSCOPIC IMAGING METHOD OF PHASE CONTRAST AND DIFFERENTIAL INTERFERENCE CONTRAST BASED ON THE TRANSPORT OF INTENSITY EQUATION

摘要

The invention claims a microscopic imaging method of phase contrast (PC) and differential interference contrast (DIC) based on the transport of intensity equation (TIE). Firstly, three intensity images are captured along the optical axis; secondly, TIE is solved by deconvolution to obtain the quantitative phase; then, the intensity image under the DIC imaging mode is obtained according to the DIC imaging principle; finally, the corresponding phase image of PC imaging mode is obtained according to the PC imaging principle. The proposed approach can endow the bright-field microscope with the ability to realize PC and DIC imaging without complex modification of the traditional bright-field microscope. In other words, this method only needs to use the traditional bright-field microscope without adding any complex hardware. Through the PC and DIC algorithms, this method has the advantages of quantitative, high-speed, low-cost, simple structure, and less external interference. In addition, it has the same imaging performance as the phase contrast microscope and differential interference contrast microscope, which are expensive, complex-structure, and has strict environmental conditions.