Method for preparing floating culture concomitant adhesive cell, method for inducing epithelial mesenchymal transition of adhesive epithelial cell and use thereof

摘要

The present invention has a step of culturing adherent cells in contact with an alicyclic structure-containing polymer molded product, a method for preparing adherent cells adapted to suspension culture, and adapted to the suspension culture by this method. Method for producing nucleic acid or protein encoded by foreign gene by culturing adherent cell, suspension culture acclimatization promoter for adherent cell comprising alicyclic structure-containing polymer molding, adherent cell in liquid medium Use of an alicyclic structure-containing polymer molded body for acclimatization to suspension culture, an adhesion-type cell culture container for adhesion type cells, at least the bottom surface comprising an alicyclic structure-containing polymer molded body, A method of inducing epithelial-mesenchymal transition having a step of culturing in a state in contact with an alicyclic structure-containing polymer molded product, by culturing adhesive cells that have undergone epithelial-mesenchymal transition by the above-described induction method, It is encoded nucleic acid or a method of producing the protein such as by coming gene.