METHOD FOR ANALYZING A BLOOD SAMPLE FROM A HUMAN FOR A TUBERCULOSIS DISEASE BY DETECTION OF TB ANTIGEN-STIMULATED CD154 EXPRESSION IN COMBINATION WITH CD38, KI-67 OR HLA-DR

摘要

The invention relates to a method for analyzing a blood sample (100) from a human for a tuberculosis disease based on tuberculosis bacteria, comprising the following steps: - providing cells (110) from the blood sample (100), - stimulating the provided cells (110) with tuberculosis antigens for induction of at least one presence marker (PM) on T cells of the provided cells (110), - labeling at least one of the induced presence markers (PM) on T cells in the provided cells (110), which marker is specifically formed on T cells which recognized tuberculosis antigen during the stimulation, - labeling at least one status marker (SM) on T cells in the provided cells (110), which marker is specific for the activation status thereof and differs from at least one presence marker (PM), - evaluating the labeling outcome of the labeling steps by means of analysis of the frequency of T cells of the provided cells (110) having labeled status marker (SM) and labeled presence marker (PM) or by means of analysis of the labeling intensity of the status marker (SM) in the provided cells (110) having labeled presence marker (PM) and comparison with a combination limit (CL).