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CULTURE MEDIUM FOR PRIMARY CELLS OF ESOPHAGEAL SQUAMOUS CARCINOMA, AND CULTIVATION METHOD THEREFOR
CULTURE MEDIUM FOR PRIMARY CELLS OF ESOPHAGEAL SQUAMOUS CARCINOMA, AND CULTIVATION METHOD THEREFOR
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机译:食管鳞状癌原代细胞的培养基,以及其栽培方法
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摘要
Provided are a culture medium and cultivation method for rapidly expanding primary cells of esophageal squamous carcinoma in vitro, and the use thereof in screening drugs. The culture medium comprises an initial culture medium selected from DMEM/F12, DMEM, F12, or RPMI-1640, a Rho protease inhibitor, an antibiotic, insulin, an N2 additive, insulin-like growth factor 1, a non-essential amino acid, and optionally, hydrocortisone, optionally, glutamine, and optionally, bovine pituitary extract.
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