NUTRIENT MEDIUM FOR MICROCLONAL REPRODUCTION OF CONTINENTAL ARALIA CONTINENTALIS KITAG

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to the field of biotechnology. The claimed nutrient medium contains half the concentration of salts dissolved in distilled water according to MS: ammonium nitrate, potassium nitrate, calcium chloride, potassium dihydrophosphate, magnesium sulfate, boric acid, manganese sulfate dihydrate, cobalt chloride, copper sulfate pentahydrate, sodium molybdenum dihydrate, zinc sulfate heptahydrate, potassium iodide, iron (II) sulfate heptahydrate, 2-water disodium salt of ethylenediaminetetraacetic acid, vitamins pyridoxine and thiamine, as well as sucrose and agar. In addition, ascorbic acid is additionally introduced into it at the following ratio of ingredients, mg/l: NH₄NO₃ 825.0; КNО₃ 950.0; CaCl₂ 166.1; KH₂PO₄ 85.0; MgSO₄ 90.35; H₃BO₃ 3.1; MnSO₄×2H₂O 8.45; CoCl₂ 0.0125; CuSO₄×5H₂O 0.0125; Na₂MoO₄×2H₂O 0.125; ZnSO₄×7H₂O 4.3; KI 0.415; FeSO₄×7H₂O 13.9; Na₂EDTA×2H₂O 18.63; pyridoxine 0.4-0.6; thiamine 0.4-0.6; ascorbic acid 0.4-0.6; sucrose 20000; agar-agar 4000; distilled water - up to 1 l. ;EFFECT: proposed nutrient medium expands the range of nutrient media that allow preserving the genotype of the original plants and further using them as pharmacological raw materials, reduces the duration of the full cycle of obtaining regenerating plants Aralia continentalis Kitag and, as a result, reduces material and labor costs. ;1 cl, 3 tbl