Method for measurement of the spontaneous aggregation of platelets in platelet-rich citrate plasma

摘要

1499435 Measuring platelet aggregation B BRAUN MELSUNGEN AG 21 Feb 1975 [21 Feb 1974] 7346/75 Heading G1A [Also in Division G2] A method of doing this comprises placing citrated plasma in a cuvette 3 and rotating it in a vertical plane at 10 to 60 R.P.M. while passing a beam from a source 2a through a lower quadrant of the cuvette to a photo-cell 4. An aggregationinhibiting drug may have been added in vitro or to a subject. Cuvette 3 of 18 mm inside and 20mm outside diameter is filled with platelet-rich blood plasma. An optical characteristic is measured, usually optical density. Filter 5 passes 500 to 650 nm preferable 546 nm. The aperture diameter of diaphragm 6 is 0.5 to 3mm, preferably 2mm. The light passes through the lower right-hand quadrant as seen from the photo-cell. Cuvette 3 is held by O-ring 8 in drum 7 preferably rotated at 20 r.p.m. Metal block 10 is heated by a resistance unit and has a thermostat with an N.T.C. resistor to maintain the temperature at 37Œ05‹C. One type of cuvette Fig. 3, (not shown) is made of glass polymethylmethacrylate or polystyrene. Another, a multi-cuvette Fig. 4, (not shown) may be of P.V.C. with two removable cover discs of polymethylmethacrylate, siliconized glass or plastics. For measuring inductive aggregation, blood plasma is mixed in siliconized glass or plastics tube 21, 9-11 mm in diameter, stirred by magnet 23, 5mm long and 2mm in diameter coated with polytetrafluorethylene driven by magnet 22 rotating at 500-1000 r.p.m., preferably 800 r.p.m. Details of preparing platelet rich citrated plasma, of which 0.5 to 1ml are used, are given. Aggregation inducers, such as adenosine diphosphate, collagen, thrombrin or adrenalin are added to the platelet suspension before rotation, as are inhibiting drugs. A potentiometer and recorder may be used to measure the signals from the photo-cell.