首页>
外国专利>
IMPROVEMENT IN YEAST EXPRESSION VECTOR AND PRODUCTION OF USEFUL SUBSTANCE USING TRANSFORMANT CONTAINING IMPROVED EXPRESSION VECTOR
IMPROVEMENT IN YEAST EXPRESSION VECTOR AND PRODUCTION OF USEFUL SUBSTANCE USING TRANSFORMANT CONTAINING IMPROVED EXPRESSION VECTOR
展开▼
机译:使用含有改良表达载体的转化子改善酵母表达载体并生产有用的物质
展开▼
页面导航
摘要
著录项
相似文献
摘要
PURPOSE:To obtain an yeast expression vector, having a sequence of specific base pairs and/or positive regulator gene GAL4 within a peripheral region on the 3'-side of an yeast gene GAL7 gene as a transcription end sequence and capable of increasing production efficiency of a desired protein. CONSTITUTION:A modified yeast expression vector obtained by integrating a transcription end sequence consisting of part or all of 328 base pairs surrounded by each cleavage site of SalI and XbaI within a peripheral region on the 3'-side of GAL7 gene and/or positive regulator gene GAL4 into an expression vector utilizing a control region of the GAL7 gene. The transcription end sequence and GAL4 gene are linked to the downstream side of the gene to be expressed. A host yeast (preferably Saccharomyces cerevisiae and relative species) is transformed with the above-mentioned expression vector linked to an exogenote and used to express an optional polypeptide.
展开▼