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Method of removing sodium dodecyl sulfate from sodium dodecyl sulfate solubilised protein solutions
Method of removing sodium dodecyl sulfate from sodium dodecyl sulfate solubilised protein solutions
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机译:从十二烷基硫酸钠溶解的蛋白质溶液中去除十二烷基硫酸钠的方法
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摘要
Guanidine hydrochloride (GCl) is used to remove excess sodium dodecyl sulfate (SDS) from SDS-solubilized protein solutions, and particularly from SDS-solubilized inclusion body solutions. GCl is added to the solution containing SDS to induce the formation of a GCl-SDS complex (GDS) which, when allowed to precipitate, can easily be removed by centrifugation, filtration, or other suitable means.
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