Determination method utilizing reagents covalently labelled with essentially non-fluorescent lanthanide chelates in combination with time- resolved fluorescence spectroscopy and the reagents to be used in the method

摘要

Compound having the following structure: ##STR1## R is a direct chain or branched alkylene group comprising 2-8 carbon atoms, PP n and m are 0 or 1,PPY is a carboxylic or phosphonic acid, andPPX is an active functional group which permits covalent coupling to a bio-organic molecule.