DNA CONSTRUCT FOR THE EXPRESSION OF ACTIVE HUMAN COPPER/ZINC SUPEROXIDE DISMUTASE IN UNICELLULAR ORGANISMS,ACTIVE ACETYLATED OR NON-ACETYLATED HUMAN COPPER/ZINC SUPEROXIDE DISMUTASE PREPARED IN UNICELLULAR HOSTS CONTAINING THE DNA CONSTRUCT AND METHODS FOR THEIR PREPARATION

摘要

Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation.;E. coli strain D1210 (pSODX8) was deposited at the A.T.C.C. on September 27, 1983 and given Accession No. 39453. Yeast strain 2150-2-3 (pC1/1GAPSOD) and E. coli strains D1210 (pSOD11) and D1210 (pS20R) were deposited at the A.T.C.C. on May 9, 1984, and given Accession Nos. 20708, 39679 and 39680, respectively.