RECOMBINANT PLASMID DNA PZAT1 USED FOR PREPARING DNA-PROBE WHICH IS USED FOR IDENTIFICATION OF TOXIGENIC STRAINS OF ANTHRAX PATHOGEN, METHOD OF CONSTRUCTION OF PLASMID DNA PZAT1 AND A STRAIN OF BACTERIUM ESCHERICHIA COLI USED FOR PREPARING RECOMBINANT PLASMID DNA PZAT1 AND DNA-PROBE WHICH IS USED FOR IDENTIFICATION OF TOXIGENIC STRAINS OF ANTHRAX PATHOGEN

摘要

FIELD: medicinal biotechnology. SUBSTANCE: method involves construction of recombinant plasmid DNA carrying species-specific fragment of anthrax plasmid pXOI which is used for DNA-probe. Synthesis level of recombinant plasmid in cells of strain is 1.5-2 mg per 1 l nutrient medium. DNA-probe prepared on the basis of plasmid DNA pZAT1 is hybridized only with toxigenic strains of anthrax pathogen that ensures to differentiate their from other bacteria including closely related bacilli and avirulent strains Bacillus anthracis no containing plasmid pXOI. EFFECT: construction of plasmid DNA indicated above. 4 cl, 1 dwg, 1 tbl