Capillary electrophoresis method using initialized capillary and polyanion-containing buffer and chemical kit therefor

摘要

A capillary electrophoresis detection and/or analysis method comprising the following steps:PPrinsing a ready-to-use capillary with an initiator to create an initialized capillary,P Padding a capillary buffer into the initialized capillary, PPinjecting a sample to be analyzed (possibly diluted with a sample diluent) into the initialized capillary,PP optionally, adding a cathodic buffer to the cathodic end of the capillary, andPPsubmitting the sample to capillary electrophoresis. PPIn this method, the capillary and/or cathodic buffer comprise(s) a polyanion or a mixture of polyanions. The polyanion or the mixture of polyanions is included at least in the capillary or the cathodic buffer. The method can additionally comprise rinsing the capillary with NaOH after electrophoresis.PPThe present invention also includes a capillary electrophoresis detection and/or analysis unit, including an initiator and a capillary buffer and/or a cathodic buffer. The capillary and/or the cathodic buffer contain a polyanion or a mixture of polyanions.