A fusion protein Tioredoxina - Human glutamate decarboxylase 65 and inmunoquimicamente Enzymatic Active, the procedure for obtaining the Recombinant Fusion Protein from E.Coli, the same Protein including Biotin, and the methods that apply these Measuring products in human serum Antibodies against glutamate decarboxylase,Useful in the Early Detection of insulin dependent diabetes mellitus and forms of autoimmune diabetes in adults

摘要

A New Protein characterized by a company formed by the merger of recombinant Hybrid proteins and Tioredoxina (TRX).Coli, and the Enzyme glutamate decarboxylase (GAD65) 65 Human, which has a sequence of adminou00e1cidos, from n-terminus to c-terminus,Corresponding to a polypeptide Chain composed of the Protein (TRX) and Tioredoxina.Coli, a connection with the decapeptide sequence (Gly ser) 2gly 4lys (ASP), and to dichaprotein Human glutamate decarboxylase 65 (GAD65) without the initial methionine.It also describes the fusion protein, containing on average 20 Molecules of biotin. The New Protein is obtained the coding sequence of the ligand in a suitable vector gad65humana Dee.Coli, Transforming the Vector into E. coli bacteria and the bacteria growing in an environment of suitable Induction, Isolating AND PURIFYING proteinaen One stage.The New Protein is obtained establebajo certain storage conditions and allows their use in New Methods for the Measurement of anti Gad autoantibodies in human serum.Which is another object of the present Invention and based enlapreincubaciu00f3n human serum to analyze with the fusion protein.