Water-soluble extract of asiaticoside and madecassoside from centella asiatica and isolating method thereof

摘要

A water-soluble extract of asiaticoside and madecassoside from Centella asiatica(L) Urb. and an isolating method thereof. Centella asiatica is immersed in an alcohol solution in water, to give an extract solution which is, then, treated with a halogenide solvent. The aqueous layer was extracted with a higher. Subsequently, the extract in the alcohol layer was washed several times with an aqueous alkaline solution, then with water, concentrated in vacuo, and crystallized with ethyl acetate. A test using a rat whose liver cells had been damaged showed that the water-soluble extract could restore them up to 40-60 %. In addition, the aqueous mix was found to relieve the fibrosis of liver cell by a bile duct ligation and liver fibrosis test.