Triple-stranded DNA, method of forming the same and southern hybridization

摘要

The present invention provides methods of preparing a triple-stranded DNA molecule by forming a DNA and protein complex, which DNA has a linear double-strand molecule and a single-strand molecule where the linear single-stranded DNA is complementary to a 5 end region of one strand in the linear double stranded DNA; and the protein component is a homogeneous recombinant protein and an exonuclease; and then the protein is removed from the complex; triple-stranded DNA; and methods of using the triple-stranded DNA to detect nucleic acid sequences.