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Method for performing multiple recombination events in a genetic system, useful e.g. for removing antibiotic resistance genes, uses mutant recombinase recognition sites
Method for performing multiple recombination events in a genetic system, useful e.g. for removing antibiotic resistance genes, uses mutant recombinase recognition sites
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机译:在遗传系统中进行多个重组事件的方法,例如用于去除抗生素抗性基因,使用突变重组酶识别位点
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摘要
Use of two non-identical recognition sequence mutants (A) for a sequence-specific recombinase (B) for performing two or more recombination events, mediated by (B), in a single genetic system. Use of two non-identical recognition sequence mutants (A) for a sequence-specific recombinase (B) for performing two or more recombination events, mediated by (B), in a single genetic system. Each (A) has two recognition sequences (RS), separated by a spacer. One RS in each (A) carries mutations that inversely repeated relative to each other, while the other RS has the wild-type sequence. Independent claims are also included for the following: (1) Method for performing multiple (B)-mediated recombination events in a single genetic system using (A); and (2) Six specific nucleic acid sequences that represent loxP mutants with flanking sequences.
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