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METHOD FOR PREPARING TEST-SYSTEM FOR DETERMINATION OF HELICOBACTER PYLORI CYTOTOXIN-ASSOCIATED PROTEIN ANTIGENS IN BIOLOGICAL MATERIAL IN INFECTED PATIENTS BY CO-AGGLUTINATION REACTION
METHOD FOR PREPARING TEST-SYSTEM FOR DETERMINATION OF HELICOBACTER PYLORI CYTOTOXIN-ASSOCIATED PROTEIN ANTIGENS IN BIOLOGICAL MATERIAL IN INFECTED PATIENTS BY CO-AGGLUTINATION REACTION
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机译:凝集反应法测定感染患者生物材料中幽门螺杆菌细胞毒素相关蛋白抗原的测试系统的制备方法
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FIELD: medicine, immunology.;SUBSTANCE: invention can be used in diagnosis of diseases associated with H. pylori using co-agglutination reaction. As material for preparing anti-toxic rabbit helicobacter serum the homogenate from chicken embryos infected with the strain H. pylori NCTC 11637 (VAC-A+, CAG-A+) and died for two days is used. Prepared serum contains mainly antibodies raised to large-molecular proteins (above 90 kDa), among them, to cytotoxin-associated CAG-A protein exhibiting high immunogenicity. On the basis of this serum Hp-tox-diagnosticum (test-system) is made for carrying out the co-agglutination reaction on plates that shows high sensitivity and specificity. Diagnosticum allows detecting antigens of large-molecular proteins in H. pylori, among them, proteins VAC-A and CAG-A being in human biological material directly (feces, saliva, circulating immune complexes). The application of method provides simplifying and accelerating the preparing test-system for carrying out the co-agglutination reaction.;EFFECT: improved preparing test-system.;2 cl, 11 dwg, 2 tbl, 6 ex
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