Proteins of the ubiquitin proteosome metabolic pathway, useful as targets for identifying fungicides for use in plant protection, also new nucleic acids, proteins and fungicides

摘要

Use of proteins (I) of the ubiquitin proteosome metabolic pathway as targets for fungicides. (I) are encoded by: (a) any of 9 sequences (X; 444-1266 bp), reproduced; (b) sequences (Y) derived by reverse translation, within the degeneracy of the genetic code, from 9 protein sequences (Z), reproduced; or (c) functional equivalents of (a) or (b), having at least 60% identity. Independent claims are also included for the following: (1) new nucleic acid sequences (IIa) that are (X), (Y) and functional equivalents having 65-86% identity with (X) or 71-98% identity with (Y), with the precise minimal degree of identity being specified for each sequence, individually; (2) polypeptides (Ia) encoded by (IIa); (3) method for detecting functional analogs of (X) by preparing a probe and then screening a genomic or cDNA library from the appropriate species, or by computer-based searching for analogs in electronic databases; (4) method for identifying mutations in (I); (5) expression cassette (EC) comprising control sequences linked to (IIa) and/or additional functional elements; (6) vector that contains EC; (7) non-human transgenic organism (bacterium, yeast, fungus or animal or plant cell) that contains (IIa), EC or the vector of (6); (8) method for identifying fungicides; (9) carrier for use in the new methods comprising (IIa), vectors of (6), hosts of (7) and (Ia); and (10) fungicides identified by method (8).