Method of Hybrid Seed Production of maize

摘要

A procedure of production and Propagation of Seedlings of maize homozygous for a Transgene that confers Male Sterility, useful for the production of hybrid Seeds of Corn.Claim 1: production process of Corn Seeds homozygous for a Transgene that confers Artificial Nuclear Male Sterility ("AMS") and heterozygous for a Fertility Restoration Gene linked to a Marker phenotype ", Small grainCharacterized in that it comprises steps consisting in: (a) Cross a Seedling of maize Male sterile heterozygous for the Transgene AMS Maize Seedling with a Fertility Restorer Gene in their Genome includes a Restoration of Fertility linked to a marker of feno "Small grain type(b) select the "Small grain phenotype" maize Seeds comprising in their Genome a Fertility Restoration Gene linked to a Marker phenotype "Small grain", (c) autofecundar Maize Seedlings arising from seeds selected according to step (b),(d) select Seeds homozygous for the Transgene AMS and heterozygous for the Fertility Restoration Gene linked to a Marker phenotype "Small grain".Claim 2: production process of Corn Seeds homozygous for a Transgene that confers Artificial Nuclear Male Sterility ("AMS") and heterozygous for a Fertility Restoration Gene linked to a Marker phenotype ", Small grainCharacterized in that it comprises steps consisting in: (a) Cross a Seedling of maize Male sterile heterozygous for the Transgene AMS Maize Seedling with a Restorer of Fertility that includes in its genome a Gene of Fertility Restoration of fenot linked to a Marker IPO "Small grain(b) performing genotyping of Seeds obtained by Crossing according to step (a), (c) autofecundar Maize Seedlings arising from seeds genotipeadas according to step (b), (d) select Seeds homozygous for the Transgene AMS and heterozygous for the gene Restoration of Fertility linked to a Marker phenotype "Small grain".Claim 3: Maize Seed homozygous and heterozygous for the Transgene AMS for Fertility Restoration Gene linked to a Marker phenotype, characterized by "Small grain" can be obtained by the method according to claim 1 or 2.Claim: in 20 Special vector, Plasmid, characterised in that it contains at least one expression cassette as described in one of claims 1 to 19. Claim 21: host Cell, wherein is especially bacteria such as Agrobacterium tumefaciens, transformed by a Vector according to claim 20.Claim 22 wherein: cell of maize is transformed by at least a Vector according to claim 20. Claim 23: Seedling Maize Restorer of Fertility which comprises in its genome, a Fertility Restorer Gene linked to a Marker phenotype "Small grain".Claim 24: Seedling Maize Transgene homozygous and heterozygous for an AMS for Fertility Restoration Gene linked to a Marker phenotype, characterized by Small grain "is derived from a SEED according to claim 3.