Method of preparing langerhans cell from CD14-positive cell being human peripheral-blood mononuclear cell with use of notch ligand delta-1, GM-CSF and TGF-beta

摘要

This invention provides a method for preparing Langerhans cells from CD14-positive cells, which are human peripheral blood mononuclear cells. This method comprises culturing human peripheral blood mononuclear cells in the presence of Notch ligand, GM-CSF, and TGF-².