Method for in vitro determination of cellular uptake of exogenous substances by means of shift agents and magic angle spinning nuclear magnetic resonance

摘要

The present invention deals with a method for in vitro quantitative determination of cellular uptake of exogenous substances characterized in that it comprises applying MAS-NMR spectroscopy to an in vitro cellular sample, in combination with a shift agent, and which includes the following steps:1) selection of the shift agent (SA) and nucleus combination to be employed for the measurement of cellular uptake of the exogenous substance (EXO) under investigation;2) determination of the cellular compartment/s (CC/s) in which said exogenous substance (EXO) distributes;3) measurement of said exogenous substance (EXO) compartmental concentration, i.e. of said EXO cellular uptake.