NEW PRIMER PAIR FOR AMPLIFYING BETWEEN ITS REGIONS FROM 16SrRNA GENE OR 16SrRNA GENE OF POTATO SCAB-CAUSING STRAIN, AND METHOD FOR DETECTING AND IDENTIFYING POTATO SCAB-CAUSING STRAIN WITH THE NEW PRIMER PAIR

摘要

PPROBLEM TO BE SOLVED: To provide a primer pair which is used for PCR and can strain-specifically amplify the genome DNAs of potato scab-causing strains, and to provide a method by which the specification of a strain in the speck tissues of scab-diseased tubers, the specification of the presence or absence of the scab strain and the strain in a potato field, and the specification of cultured scab fungi can quickly and simply be carried out at low costs. PSOLUTION: This primer pair for PCR hybridizes to a strain-specific base sequence of a 16SrRNA gene of the scab strain and a 16S-23S internal transcribed spacer (ITS), wherein DNA fragment sizes (base pairs) to be amplified are clearly different among the strains. A method for amplifying a genome DNA between hybridized primers, and a method for identifying an amplified product. PCOPYRIGHT: (C)2006,JPO&NCIPI