METHOD FOR IMMUNOENZYME ANALYSIS FOR ASSAY OF VON WILLEBRAND FACTOR, MONOCLONAL ANTIBODY TO VON WILLEBRAND FACTOR (VARIANTS) AND STRAIN OF HYBRID CULTURED MAMMAL CELLS MUS MUSCULUS L - PRODUCER OF MONOCLONAL ANTIBODIES TO VON WILLEBRAND FACTOR (VARIANTS)

摘要

FIELD: immunology.;SUBSTANCE: invention relates to immunoenzyme analysis and can be used for assay of von Willebrand factor. Method involves immunoenzyme analysis wherein monoclonal antibody 5C3 is used as an immobilizing antibody, and a mixture of biotin-labeled monoclonal antibodies 2H2 and 7D12 is used as a detecting antibody. Also, invention relates to monoclonal antibodies produced by the strain of hybridoma cultured cells Mus musculus L. and directed against von Willebrand factor, and to strains of hybrid cultured cells Mus musculus L. producing indicated monoclonal antibodies. Invention provides the development of highly sensitive method for assay of von Willebrand factor.;EFFECT: improved method for analysis.;9 cl, 1 tbl, 2 dwg, 3 ex