GENOME OF LEGIONELLA PNEUMOPHILA SOUCHE PARIS- DIAGNOSTIC AND EPIDEMIOLOGICAL APPLICATIONS

摘要

Isolated or purified nucleotide sequences (I) from Legionella pneumophilaParis strain, that: (a) is any of 56 sequences (not reproduced but available from the French Patent Office); (b) has at least 80% sequence identity with (a); (c) hybridizes under stringent conditions to (a); (d) is the complement of (a)-(c), or is the corresponding RNA; or (e) comprises at least 15 nucleotides from (a). Independent claims are also included for the following: (1) nucleotide sequences (Ia) that include a sequence (Ib) encoding any of 3399 polypeptides (II), also sequences related to (Ib) in the same way that (b)-(e) are related to (a) above; (2) polypeptides (II) encodes by (I) or (Ia); (3) polypeptide (IIa) that comprises (II), or sequences with at least 80% identity or biologically active fragments with at least 5 amino acids; (4) nucleotide sequences (Ic) that encode (IIa); (5) DNA chip or filter that includes, as primer or probe, at least one (I), of (b)-(e), (Ia) or (Ic); (6) kit for detecting/identifying Legionella, at the level of genus, species or strain, containing the chip or filter of (5) or any of the sequences present on the chip or filter; (7) cloning and/or expression vector containing a (I), of (b)-(e), (Ia) or (Ic); (8) host cell transformed with the vector of (7); (9) plant or non-human animal containing a cell of (8); (10) method for preparing a polypeptide (PP) by culturing cells transformed by the vector of (7); (11) recombinant PP prepared by method (10); (12) preparation of (II) or (IIa) by chemical synthesis; (13) hybrid polypeptide (hPP) containing (i) at least one (II) or (IIa) that is a surface polypeptide of L. pneumophilaand (ii) the sequence of a polypeptide (III) able to induce an immune response in a human or animal; (14) nucleic acid sequence (Id) that encodes hPP; (15) vector containing (Id); (16) mono- or poly-clonal antibodies (Ab), or their fragments, or chimeric antibodies able to recognize specifically (II) or (IIa); (17) method for detecting/identifying Legionella, at the level of genus, species or strain, by using Ab and detecting any complex formed; (18) method for detecting and/or quantifying expression of a specific gene in Legionellaby using Ab and detecting any complex formed; (19) kit for performing methods (17) and (18); (20) protein chip that contains at least one of the new polypeptides or Ab, immobilized on a support; (21) kit for detecting/identifying Legionella, at the level of genus, species or strain, containing the chip of (20); (22) method for detecting/identifying Legionella, at the level of genus, species or strain, by using any of the new nucleotide sequences; (23) kit for detecting/identifying Legionella, at the level of genus, species or strain, that includes the primer/probe of (6), optionally also reagents for performing hybridization or amplification assays; (24) method for selecting a compound that inhibits expression of genes implicated in biosynthesis of envelope polysaccharides in L. pneumophilaParis strain; (25) vaccinating or immunogenic composition that contains (a) (II) or (IIa) that are surface proteins of L. pneumophilaParis strain or hPP or (b) a vector of (7) or (15); (26) genomic DNA bank from L. pneumophilaParis strain, deposited as CNCM I-3138; and (27) method for isolating selected polynucleotides by using the bank of (26). ACTIVITY : Antibacterial. No details of tests for antibacterial activity are given. MECHANISM OF ACTION : Vaccine; inhibiting expression of genes involved in synthesis of surface polysaccharides.