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How to identify Japanese flounder resistant to lymphocystis disease

机译:如何鉴定对淋巴囊病有抗性的日本比目鱼

摘要

PPROBLEM TO BE SOLVED: To discriminate a flatfish resistant to lymphocystis disease by using a specific marker. PSOLUTION: The method for the discrimination of the flatfish resistant to lymphocystis disease comprises the PCR reaction of the genom DNA of the flatfish using a specific oligonucleotide selected from microsatellite marker exhibiting the polymorphism of flatfish as a primer, and the confirmation of the presence of bands of 101 bp, 130 bp or 106 bp in the gel electrophoresis of the PCR product. PCOPYRIGHT: (C)2004,JPO
机译:

要解决的问题:通过使用特定标记来区分对淋巴囊虫病有抵抗力的比目鱼。

解决方案:辨别对淋巴囊虫病具有抵抗力的比目鱼的方法包括:使用选自表现出比目鱼多态性的微卫星标记的特异寡核苷酸作为引物,对比目鱼基因组DNA进行PCR反应,并确认PCR产物的凝胶电泳中存在101 bp,130 bp或106 bp的条带。

版权:(C)2004,日本特许厅

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