Method for producing animal model for heart failure test causing left ventricular diastolic disorder and method for testing drug for treating heart failure due to left ventricular diastolic failure using said animal model

摘要

An animal model capable of performing a drug efficacy test for left ventricular myocardial dysfunction, for example, heart failure due to left ventricular diastolic dysfunction is provided. Inject an aqueous solution of calcium chloride into the vein of an anesthetized small laboratory animal, and continue to inject the aqueous solution of calcium chloride into the vein, injecting an aqueous solution of norepinephrine into the vein to expand the left ventricle of the animal's heart. A method for producing an animal model for testing a therapeutic agent for heart failure that causes left ventricular diastolic dysfunction characterized by increasing the end-stage pressure to a level higher than the normal left ventricular end-diastolic pressure, and the therapeutic agent for heart failure produced by this method Intravenous norepinephrine aqueous solution is injected into the vein of the test animal model to measure left ventricular end diastolic pressure, and the measured left ventricular end diastolic pressure is compared with normal left ventricular end diastolic pressure before infusion of norepinephrine aqueous solution There is a method for testing a therapeutic agent for heart failure caused by left ventricular diastolic dysfunction using an animal model for heart failure testing in which left ventricular diastolic disorder has occurred.