Method to study bomolecular interactions under native condition by MALDI

摘要

A sample preparation method is disclosed for volatilization and mass spectrometric analysis of nonvolatile high molecular weight molecules. Buffered aqueous solutions of matrix at near physiological pH are disclosed for use in Matrix-assisted Laser Desorption and Ionization (MALDI). The new methods expanded the application of MALDI to study non-covalent protein complexes.