Expression vectors Co1E1 origin of replication modificaco to control the number of copies of plasmids.

摘要

An expression vector having a replication system ColE1, wherein the homology of the RNAI and RNAII of the ColE1 origin of replication with uncharged tRNAs is modified by one or more mutations in the coding region of the RNAI gene and one or more corresponding mutations in the gene Ranii, said mutation producing one or more base exchanges in loop 1 and / or loop 2 and / or loop 3 of RNAI and RNAII.