LIQUID COMPOSITION OF MICROORGANISMS FOR BIOREMEDIATION OF HYDROCARBON-CONTAMINATED SOIL, METHOD OF PREPARING THE SAME, AND BIOREMEDIATION USING THE SAME

摘要

A liquid composition of microorganisms for remediation of oil-contaminated soil is provided to increase degradation activities of oil, a method of preparing the composition is provided, and a method for prompt bioremediation of oil-contaminated soil using the composition is provided. A liquid composition of microorganisms for remediation of oil-contaminated soil is characterized in that a culture fluid of a high growth strain which reaches a stationary phase within 10 hours having degradation ability of oil separated from oil-contaminated soil and a culture fluid of a low growth strain with a growth rate slower than that of the high growth strain are mixed with each other. A method of preparing a liquid composition of microorganisms for remediation of oil-contaminated soil comprises the steps of: sorting and selecting a strain with oil degrading ability from oil-contaminated soil; separating a strain reaching a stationary phase within 10 hours as a high growth strain, and separating a strain reaching the stationary phase after 10 hours as a low growth strain according to the growth rate of the strain; culturing the high growth strain and the low growth strain in respective culture media; and mixing a culture fluid of the high growth strain with a culture fluid of the low growth strain. Further, 0.001 to 1 g of enzyme expression accelerator is comprised based on 1L of media in the culture media.