首页>
外国专利>
METHODS OF DESIGNING SHORT HAIRPIN RNAS (SHRNAS) FOR GENE SILENCING
METHODS OF DESIGNING SHORT HAIRPIN RNAS (SHRNAS) FOR GENE SILENCING
展开▼
机译:设计用于基因沉默的短发RNA(SHRNA)的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The invention relates to methods and apparatuses and computer program products for classifying siRNA functional sequence motifs for use in an shRNA core sequence, and for designing shRNA, shRNA core sequences or sense strand sequences thereof or antisense sequences thereof. The shRNA core sequence comprises an antisense strand sequence and a sense strand sequence and is designed to produce an siRNA for silencing a target gene in a cell. In one embodiment, the method selects a first plurality of siRNA functional sequence motifs each with a predicted silencing efficacy against a targeted sequence motif within a transcript of a target gene that is equal or greater than a chosen threshold of silencing efficacy, and classifies each siRNA functional sequence motif in the first plurality of siRNA functional sequence motifs as belonging to a first class of siRNA functional sequence motifs when the siRNA functional sequence motif has a 3 ' terminal nucleotide that is a cytosine, or as belonging to a second class of siRNA functional sequence motifs when said siRNA functional sequence motif does not have a 3 ' terminal nucleotide that is a cytosine. The invention further discloses methods of designing by replacing the 3 ' terminal non-cytosine nucleotide with a cytosine or by adding a 3 ' terminal cytosine to an siRNA functional sequence motif.
展开▼