PRODUCTION OF THE ANTIGENIC EPITOPE RECOMBINANT PROTEIN OF HELICOBACTER PYLORI FLAA REACTIVE TO HUMAN ANTISERA AND ITS METHOD

摘要

An epitope recombinant protein which is reactive to human antisera and producing method thereof are provided to improve health care of stomach and duodenum by widely develop the diagnosis method. A method for manufacturing an epitope recombinant protein which is reactive to human antisera of FlaA of helicobater pylori comprise: a step of amplifying antigen gene and epitope partial gene fragment; a step of treating with a restriction enzyme; a step of isolating and purifying the cut gene; a step of treating with same restriction enzyme in a vector; a step of expressing protein and mapping; and a step of expressing a clone and obtaining the expressed protein.