Culture medium for propagation of plants of pineapple (Ananas comosus) in vitro; culture medium for induction of pineapple (Ananas comosus) seedlings in vitro; and raw extract of seedlings of pineapple (Ananas comous)

摘要

Culture medium for propagation of plants of pineapple (Ananas comosus) in vitro; culture medium for induction of pineapple (Ananas comosus) seedlings in vitro; and raw extract of seedlings of pineapple (pineapple like us).This is the invention that relates to the field of biotechnology and, in particular, obtaining metabu00f3lits second rivers, through induction, such as bromelain of plants of the genus Ananas spp., previously established in vitro.The aim of the invention is the development of an innovative process for extracting bromelain from pineapple natural fabrics plant established and induced in vitro with greater activity protcolu00edtica (200 to 600%) and in high concentrations, compared with the protein that is generated is Ma naturally in the plant and is currently extracted by traditional methodologies.In this process, use is made of Pineapple Seedlings established and induced in vitro, which are processed in a buffer solution of phosphate for its extraction.Thereafter, are subjected to processes of filtration, centrifugation, freezing and freeze-drying to obtain a crude bromelain extract characterized by high yields in terms of protein and specific activity.