Cytochrome P450 monooxigenase, nucleic acid sequence, construct of expressu00e7o, vector, recombinant microorganism, processes for the oxidau00c7u00e7o microbiolu00e0gica of a compound aromu00c1tico mono or polinuclear n -, - S - heterocu00cdclico and the produu00c7u00e7o microbiolu00e0gica indigo and / or indirubi N, uses of a cytochrome P450 monooxigenase,A vector or a recombinant microorganism and a microorganism which produces a cytochrome P450 oxidation of indole, and bioreactor.

摘要

"Cytochrome P450 monooxigenase, sequence of nucleic acid construct expression vector, a recombinant microorganism.Process for the microbiological oxidation of an aromatic compound of mono - or polinuclear n - or S - heterocu00ecclico, microbiological process for the production of indigo and / or indirubin, bioreactor.Use of a cytochrome P450 monooxigenase, a vector, or a microorganism, and use of a microorganism which produces cytochrome P450 oxidation of indole.The invention relates to the new cytochrome P450 substrate specificity monooxigenases comprising a modified to sequ00b3u00eancias nucleotides coding for the same.The vectors and constructos expression containing these sequ00b3u00eancias, and microorganisms transformed with the same.The invention also relates to methods for oxidizing organic microbiologically different substrates, such as methods for producing indigo and indirubina.