METHOD FOR DIFFERENTIATING YERSINIA PESTIS STRAINS OF VARIOUS SUBSPECIES AND BIOVARS BY POLYMERASE CHAIN REACTION AND MULTILOCUS SEQUENCE TYPING

摘要

FIELD: medicine.;SUBSTANCE: method provides: recovering chromosome DNA of the examined strain, conducting polymerase chain reaction with primers Nap469/Nap1187, rhaSc-s/rhaSc-a, glpD-Fl/glpD-Rl, aRaCF2/aRaCR2 with the primers of amplification genes of napA and rhaS gene fragments having the following nucleotide sequences: Nap469-TACGCGGCG TTGAAGTTG; Nap1187-TTGCCGGTT AACAGGTGC, rhaSc-s-GGTTTAGTCA TCACTGCTGC; rhaSc-a-GAAGTGCGGGAAAGAA, genetic typing by the presence of nucleotide substitutes in variable sites of the used genes, and sequence typing of the examined strain. The examined plague agent strains are differentiated by comparing their sequence types and the sequence types of the strains of primary and secondary subspecies and specific biovars of the plaque agent.;EFFECT: method enables effective and reliable intraspecific differentiation of the Yersinia pestis strains by subspecies and biovars.;1 dwg, 2 tbl