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METHOD FOR METABOLOMIC SAMPLE PREPARATION BASED ON IONIC LIQUID DISPERSIVE LIQUID-LIQUID MICROEXTRACTION
METHOD FOR METABOLOMIC SAMPLE PREPARATION BASED ON IONIC LIQUID DISPERSIVE LIQUID-LIQUID MICROEXTRACTION
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机译:离子液体分散液-液微萃取的代谢组学样品制备方法
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摘要
Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided.
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