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Method of culturing pancreatic islet-like tissues by a tissue complex of pancreas-derived non-endocrinal epithelial cells and vascular endothelial cells
Method of culturing pancreatic islet-like tissues by a tissue complex of pancreas-derived non-endocrinal epithelial cells and vascular endothelial cells
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机译:通过胰腺来源的非内分泌上皮细胞和血管内皮细胞的组织复合物培养胰岛样组织的方法
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摘要
According to embodiments, a method of producing insulin-producing tissues (IPTs) by culturing comprises: preparing non-endocrinal epithelial cells (NEECs) and vascular endothelial cells (VECs), which have been isolated or originated from postnatal pancreata, preferably by capturing of NEECs by collagen; culturing in vitro the NEECs and the VECs at least partly separately from each other; and then generating in vitro a tissue complex (IPTs) that contains both the NEECs and the VECs. In another embodiment, the native islet cells are seeded on a monolayer of VECs that have preferably been separately cultured and purified. In a further embodiment, a method of enriching NEECs comprises: culturing NEECs adhering to a container or substrate; removing NEECs by treating with a tissue-dissociation enzyme to leave left-over cells (LOCs) still attached on the container or substrate; and culturing NEECs in a medium conditioned by, or in the presence of the LOCs.
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