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Kit for incorporation of single oxygen atom into a digested peptide of a protein comprising a peptidase and enriched 18o water
Kit for incorporation of single oxygen atom into a digested peptide of a protein comprising a peptidase and enriched 18o water
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机译:用于将单个氧原子掺入包含肽酶和18o水的蛋白质的消化肽中的试剂盒
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摘要
Optimized enzymatic conditions incorporate a single oxygen atom into digested peptides using a peptidase. The incorporation of a single oxygen atom is especially useful for proteolytic 18O labeling in comparative proteomics. The optimized proteolytic 18O labeling minimizes the generation of a mixture of isotopic isoforms of the peptides resulting from incorporation of either one or two 18O atoms. The outcome is accurate quantification of isotopically labeled peptides.
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