Method for detection of cAMP and cGMP

摘要

An in vitro method for detecting the cyclic adenosine monophosphate (cAMP) or cyclic guanosine monophosphate (cGMP), comprising a) contacting a mixture comprising cAMP or cGMP with a complex of an indicator and unreactivador, wherein the indicator is a fluorophore covalently linked to a quencher cAMP cAMP eldesactivador is 2- (6-aminohexyl) amino-cAMP, 8- (6-aminohexyl) amino-cAMP, 8- (8-amino-3,6-dioxaoctilamino) - cAMP, 8-hydroxy-cAMP or 8- (4-mercaptobutiltio) -cAMP, and quencher cAMP is able dedesactivar the fluorophore which is covalently bonded, and the reactivator is capable of binding the deactivator deAMPc well as cyclic nucleotide to detecting, b) measuring the change in fluorescence, where the change of measured fluorescence is compared with a control.