Screening for compounds having immunosuppressant activity by testing impact on leukocyte-specific calcium fluxes

摘要

The invention relates to an assay for the identification of a compound having immunosuppressant activity, wherein a candidate compound is analyzed whether it blocks the Ca²⁺ flux in coronin 1 expressing cells and/or in coronin 1 negative cells. A candidate compound is identified as having immunosuppressant activity if it blocks the Ca²⁺ flux specifically in coronin 1 expressing cells. Further described are upstream assays wherein the impact of a candidate compound on coronin 1 trimerization is measured, and downstream assays wherein the impact of a candidate compound on diacyl glycerol (DAG) generation, phosphatidylinositol-4,5-biphosphate (PIP₂) levels and/or inositol-1,4,5-triphosphate (InsP₃) generation or on nuclear factor of activated T cells (NFAT) nuclear localization is determined.