METHOD FOR PREPARING ANDROGEN RECEPTOR ACTIVITY-DEPENDENT BREAST CANCER CELL LINE, SCREENING METHOD USING CELL LINE, AND METHOD, KIT AND MARKER FOR DETERMINING ACQUISITION OF ANDROGEN RECEPTOR ACTIVITY DEPENDENCE IN BREAST CANCER PATIENT

摘要

The present invention addresses the problem of providing a cell line having a novel resistance mechanism that is completely different from the resistance mechanisms in conventional hormone therapy, a screening method using the cell line, and a method for determining whether or not a breast cancer patient has acquired the aforesaid resistance mechanism.Provided are: a method for preparing androgen receptor activity-dependent breast cancer cells, said method comprising a step for culturing T47D-TE8 cells in an estrogen-depleted medium in the presence of androgen; a screening method using the breast cancer cells; and a method and a kit for determining the presence or absence of the acquisition of androgen receptor activity dependence in a breast cancer patient with the use of at least one member selected from the group consisting of KLK3, DDC and AR as an indicator.