DNA APTAMER SPECIFICALLY BINDING TO VIRULENCE REGULATORY FACTOR PRFA PROTEIN OF LISTERIA AND USE THEREOF

摘要

The present invention relates to a DNA aptamer specifically binding to the PrfA protein, which is a virulence regulatory factor of Listeria and a use thereof, and more specifically, a method for detecting Listeria monocytogenes using a DNA aptamer specifically binding to the PrfA protein of Listeria monocytogenes, and to a composition for diagnosing listeriosis. The DNA aptamer according to the present invention has activity to specifically bind to the PrfA protein of Listeria monocytogenes, and thus can be useful in early detection of Listeria monocytogenes, which is the harmful bacterium present in the conventional natural environment, food, and the human body, and early diagnose listeriosis. In addition, the DNA aptamer of the present invention has high specificity to PrfA protein of Listeria monocytogenes, thereby improving diagnostic sensitivity and mass-producing the same quality of aptamer at a low cost, and thus can be effectively used from an economical aspect.