INDUCED SMOOTH MUSCLE-TYPE CELLS, METHODS FOR THE PRODUCTION THEREOF, AND USES THEREOF IN CELL-BASED TISSUE REGENERATION AND TREATMENT OF VASCULAR OCCLUSION

摘要

An induced smooth muscle-like cell (iSMC) is described. The iSMC is prepared by reprogramming a primitive progenitor cell comprising introducing into the cell an agent capable of increasing the expression of myocardin in the primitive progenitor cell. The agent may be a nucleic acid construct comprising a KLF4 knockdown agent, an artificial myocardinspecific transactivator, or a myocardin transgene typically under the control of a transcriptional promotor. Methods of producing the cells, and the use of the cells in autologous tissue regeneration therapy or treatment of vascular occlusion in humans is also described.