Method of preparing novel poly herbal formulations containing curcumin, quercetin and piperine in the form of solid lipid nano particles and nano crystals

摘要

The present invention relates to process of preparing herbal solid lipid nanoparticles and nanocrystals loaded with curcumin, quercetin and piperine with improved bioavailability. The phyto constituents curcumin, quercetin and piperine had more therapeutic benefits but their utility was limited by poor solubility, permeability and resulted low bioavailability. Hence these constituents were characterized based on Biopharmaceutical classification system. Then herbal solid lipid nanoparticles loaded with curcumin, quercetin and piperine were prepared by micro emulsion technique with lipid glycerol monostearate and nanocrystals prepared by antisolvent recrystallization technique. In vitro characterization was performed including particle size and distribution, zeta potential, dissolution, scanning electron microscopy. The herbal solid lipid nanoparticles and nanocrystals exhibited significantly enhanced dissolution. Invivo Pharmacokinetic tests were performed in rabbits. Pharmacokinetic parameters were evaluated with reverse phase ultra fast liquid chromatography. The herbal solid lipid nanoparticles and nanocrystals had rapid and extensive absorption with improved bioavailability.